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KMID : 0385019910070020053
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Korean Journal of Laboratory Animal Science
1991 Volume.7 No. 2 p.53 ~ p.61
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Detection of Antibodies to Sendai Virus Using Protein G-based ELISA
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Abstract
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We have established an enzyme-linked immunosorbent assay(ELISA) using enzyme labeled protein G(Pratein G-ELISA) to detect Sendai virus-specific antibodies in laboratory animals. Best results were obtained at pH 6.0 of diluent and at 1 to 10 dilution of sera. Antigen concentration for solid phase matrix was 5 ㎍/㎖ and the conjugate of horseradish peroxidase (HRP) and protein G was used at 1 to 100 dilution. We, also, investigated the sensitivity and specificity of protein G and protein A. Protein G showed relatively high O. D. values than protein A in general. Furthermore, only Protein G-ELISA could detect the Sendai virus specific antibodies in rat, though both methods were effective in other species, mouse and guinea pig. No significant nonspecific reaction was observed at our experimental conditions in both methods.
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